The Eliminate Dengue Program started with lab experiments where Wolbachia
-infected mosquitoes were released in cages filled with uninfected mosquitoes and grew to include field pilot studies, of which there are now six: Australia, Indonesia, Vietnam, Brazil, Colombia, and most recently, India.
Dr. Aliota is collaborating with the Eliminate Dengue Program in Colombia. The program first released Wolbachia-
infected Aedes aegypti
mosquitoes in the Colombia site, a city in the northwest region of the country, in May 2015. Dr. Aliota stated that Wolbachia
-infected mosquitoes were released for a short period and then the bacteria were able to spread throughout the mosquito population. In October 2016, the Program announced that more than 90% of mosquitoes in the Colombia site.
In his presentation he stated that his research team, in cooperation with the Eliminate Dengue Program in Colombia, set out to discover if the wMel strain of Wolbachia
would be successful in reducing the transmission of Dengue-related viruses by Aedes aegypti
mosquitoes. They found that, in fact, this strain can successfully block viral transmission of Chikungunya and the Puerto Rican [PR] strain of the Zika virus.
The team infected mouse models with the PR strain and allowed the mosquitoes to bite them at peak viremia. Following this, the team collected mosquito tissues and tested them for Zika infection. In wild-type Aedes aegypti
, which were not infected with Wolbachia
, it was observed that “almost 100% of mosquitoes [were] infected at 4 days post-feeding but [there was] no dissemination or transmission.” The team found infection transmission in some mosquitoes at 7 days post-feeding, with the percentage of transmission steadily increasing after 7 days. On the other hand, in mosquitoes infected with the bacteria, there was “no infection dissemination or transmission” at 4 days post-feeding; at 7 and 10 days post-feeding, the virus was not detectable in mosquito saliva, meaning that there was no potential to transmit infection to the host.
Complementary results were observed in Wolbachia
-infected Aedes aegypti
with the Brazilian strain of the Zika virus. Dr. Aliota stated that “using the same experimental system” the team was able to test Wolbachia
against Chikungunya, and found similar results.
First International Conference on Zika Virus
Exclusive interview with Contagion®
Session 5A: In The Pipeline
as a Novel Approach for the Control of Arboviruses Transmitted by Aedes aegypti
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